Review of methods of amniotic membrane preservation
https://doi.org/10.17021/2712-8164-2025-3-6-11
Abstract
Methods for covering wound surfaces during surgical treatment of various diseases necessitate the use of the patient's own tissues, synthetic constructs, and allogeneic donor material. Among the latter, the amniotic membrane is attracting increasing interest from researchers. However, preserving the structure and properties of the amnion requires the use of specific preservative media and temperature regimes. In this article, we provide an overview of several methods for preserving the amniotic membrane.
About the Authors
A. M. Gaponik
Grodno State Medical University
Беларусь
Беларусь
Alexey M. Gaponik, Assistant Professo
Yu. M. Kiselevsky
Grodno State Medical University
Беларусь
Беларусь
Yuri M. Kiselevsky, Cand. Sci. (Med.), Associate Professor
References
1. Budasheev V. P., Grigoriev E. G., Lepekhova S. A., Goldberg O. A., Tsybikov E. N., Kapsalykova L. N. Methods for optimizing intestinal anastomosis in conditions of experimental widespread purulent peritonitis. Byulleten' Vostochno-Sibirskogo nauchnogo tsentra Sibirskogo otdeleniya RAMN = Bulletin of the All-Russian Scientific Center SB RAMS. 2006; 6: 139–146 (In Russ.).
2. Kosova D. A., Aleksandrov L. S., Ishchenko A. I., Trifonova N. S., Nikonov A. P., Lavrentieva K. I., Ageev M. B., Fedorova E. V., Lyndup A. V., Zholobova M. N., Berishvili M. V., Proklova G. F., Zemlina N. S., Naumenko N. S. Application of amniotic membranes in clinical medicine. Voprosy ginekologii, akusherstva i perinatologii = Issues of Gynecology, Obstetrics and Perinatology. 2023; 22 (5): 69–78 (In Russ.).
3. Gavrilyuk I. O., Aleksandrova O. I., Kuznetsova A. YU., Mashel T. V., Seleznev A. S., Chernysh V. F., Churashov S. V., Blinova M. I., Kulikov A. N. Mechanical preparation of amniotic membrane in the creation of bioengineered structures for the restoration of corneal epithelium. Vestnik Rossiyskoy Voyenno-meditsinskoy akademii = Bulletin of the Russian Military Medical Academy. 2019; 4: 116–120 (In Russ.).
4. Milyudin E. S., Kuchuk K. E., Bratko O. V. Preserved amniotic membrane in the structure of the tissue-engineered complex of the anterior corneal epithelial layer. Biologiya i eksperimentalnaya meditsina = Biology and Experimental Medicine. 2016; 33 (5): 47–53 (In Russ.).
5. Sitnik G. V., Yakhnitskaya L. K. Use of amniotic membrane in ophthalmic surgery. Meditsinskiy zhurnal = Medical Journal. 2005; 3: 119–122 (In Russ.).
6. Volkov O. E., Zavada N. V., Ladutko I. M. Experimental justification for the use of the method of suturing a small intestinal rupture with an everted mechanical suture using an amniotic membrane. Vestnik Vitebskogo gosudarstvennogo meditsinskogo universiteta = Bulletin of Vitebsk State Medical University. 2017; 16 (3) 38–45 (In Russ.).
7. Kupchenko A. M., Stanovenko V. V., Sharkova L. I. Experimental substantiation of the possibility of using amniotic membrane in surgical practice. Dostizheniya fundamentalnoy, klinicheskoy meditsiny i farmatsii = Achievements of fundamental, clinical medicine and pharmacy. Ed. by A. T. Shchastny. Vitebsk: Vitebsk State Medical University; 2020: 31–33 (In Russ.).
8. Ob utverzhdenii klinicheskikh protokolov: postanovleniye Ministerstva zdravookhraneniya Respubliki Belarus = On approval of clinical protocols. Resolution of the Ministry of Health of the Republic of Belarus. February 13, 2023; 31: 210–211. URL: https://pravo.by/document/?guid=12551&p0=W22339846p (In Russ.).
9. Voronin G. V., Osipyan G. A., Trufanov S. V., Budnikova Ye. A., Rozinova V. N., Subbot A. M., Makarova M. A. Methods of preservation of donor corneas. Vestnik oftalmologii = Bulletin of Ophthalmology. 2018; 5: 238–243 (In Russ.).
10. Sereda Ye. V., Drozhzhina G. I., Gaydamaka T. B., Vit V. V. Effect of viable cryopreserved human amniotic membrane fixed to the cornea with suture material on the course of modeled bacterial keratitis. Oftalmologicheskiy zhurnal = Ophthalmological Journal. 2015; 5: 35–41 (In Russ.).
11. Chentsova Ye. V., Borovkova N. V., Makarov P. V., Bozhenko D. A., Ponomarev I. N., Storozheva M. V., Makarov M. S. Biological effect of a combination of platelet lysate and amniotic membrane in buccal epithelial culture. Rossiyskiy oftalmologicheskiy zhurnal = Russian Ophthalmological Journal. 2022; 15: 115–120 (In Russ.).
12. Podoprigora R. N. Methods of preservation of donor material. Vestnik Orenburgskogo gosudarstvennogo universiteta = Bulletin of the Orenburg State University. 2004; 13: 100–103 (In Russ.).
13. Vasiliev A. M., Andreev A. N. Experience of using silicone-dried amniotic membrane implant “Flexamer” for the treatment of persistent corneal defects. Acta medica Eurasica. 2016; 4: 1–4 (In Russ.).
14. Ostrozhinsky Ya. A., Shust L. G., Chisty A. A. Pathophysiological aspects of various methods of cold preservation of human donor tissues. Nauchnyy meditsinskiy vestnik Yugry = Scientific Medical Bulletin of Yugra. 2021; 1 (5): 126–128 (In Russ.).
15. Iskandarov M. A. Evaluation of the regenerative activity of the amniotic membrane after preservation. XII Kongress Mezhdunarodnoy assotsiatsii morfologov i VII sezd Rossiyskogo nauchnogo meditsinskogo obshchestva anatomov, gistologov i embriologov = XII Congress of the International Association of Morphologists and VII Congress of the Russian Scientific Medical Society of Anatomists, Histologists and Embryologists. 2014; 145 (3) 83 (In Russ.).
16. Maltsev D. S., Rudko A. S., Kulikov A. N., Chernysh V. F. The effect of amniotic membrane extract on epithelialization and neovascularization in corneal damage models. Tikhookeanskiy meditsinskiy zhurnal = Pacific Medical Journal. 2018; 2: 46–49 (In Russ.).
Review
For citations:
Gaponik A.M., Kiselevsky Yu.M. Review of methods of amniotic membrane preservation. Title. 2025;6(3):6-11. (In Russ.) https://doi.org/10.17021/2712-8164-2025-3-6-11
Views:
98
JATS XML
Email this article 