Antioxidant activity of Lonicera xylosteum leaf extract

In modern medicine and pharmacy, there is a steady tendency to search for natural compounds that can level oxidative stress a key factor in the pathogenesis of diseases of various etiologies. At the moment, special attention   is paid to the study of potentially new sources of antioxidants, including plant origin. Purpose of the study: evaluation of antioxidant activity potential of Lonicera xylosteum leaf extract. Materials and methods. The object of the study was an extract of wild honeysuckle leaves. The quantitative determination of biologically active substances was carried out according to the generally accepted spectrophotometric methods indicated in the State Pharmacopoeia: flavonoids in terms of catechin, hydroxycinnamic acids in terms of chlorogenic acid, tannins in terms of gallic acid. The antioxidant potential of common honeysuckle leaf extract was assessed using a “model biological system” as a suspension of chicken egg yolk lipoproteins, determining the level of products that react with thiobarbituric acid and lipid peroxidation rates. The control sample was taken without the test extract. The reference drug was Emoxipine. Results. Quantitative analysis of the biologically active substances of the leaves of Lonicera xylosteum showed the presence of flavonoids in terms of catechin 1.17 %, hydroxycinnamic acids in terms of chlorogenic acid 0.84 % and tannins in terms of gallic 4.16 %. Under the influence of the studied extract and reference preparation, there was a decrease in the level of thiobarbituric acid reactive products and the rate of lipid peroxidation in relation to the sample with induced fatty acid oxidation by 1.4 and 1.7 times (p <0.05), as well as 1.2 and 1.4 times (p <0.05), respectively. Conclusion. The obtained results showed that   the extract of Lonicera xylosteum leaves suppresses the induced lipid peroxidation, reducing the level of products reacting with thiobarbituric acid, while the antioxidant effect is comparable to the reference preparation, which is due to the presence of a large amount of biologically active substances in the leaves, which ensure stabilization of lipoperoxidation processes. The data obtained indicate the antioxidant effect of Lonicera xylosteum leaf extract, further comprehensive study of which may be of practical importance for the creation of new pharmaceutical substances.

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