Combined antioxidant correction of toxic liver damage in the experiment

Abstract

A wide range of drugs with metabolic action have been developed for the prevention and treatment of liver damage of various etiopathogenesis. However, the efficacy of these drugs in clinical settings remains unsatisfactory, necessitating the development of new approaches to hepatoprotective therapy. Aim. The study aims at analyzing the efficacy of combination therapy for toxic liver damage in an experiment using agents with antihypoxic and antioxidant effects. Materials and Methods. Ten groups of rats were formed, with 10 individuals in each: Group 1 was a control group, the rest were subjected to acetaminophen-induced hepatitis and were administered for 10 days with the following: saline solution (comparison group), remaxol (Group 3), ascorbic acid (Group 4), α-lipoic acid (Group 5), α-tocopherol acetate (Group 6) and combinations of remaxol with antioxidants (Groups 7–10). Following the experiment protocol, blood was collected from the caudal vena cava and liver for laboratory testing. Cytolytic syndrome markers and prooxidant-antioxidant balance indicators were determined. Results. Remaxol demonstrated the ability to reduce the manifestations of cytolytic syndrome in acute toxic liver injury, which was characterized by reduced aminotransferase and lactate dehydrogenase activity by 12–25 % relative to the comparison group. Analysis of the combined effect of antioxidant drugs revealed a potentiating effect with the combined use of remaxol and antioxidants. The group of animals receiving remaxol and all 3 antioxidants was characterized by the lowest aminotransferase and lactate dehydrogenase activity values that was reduced by 2.0, 1.7, and 2.6 times, respectively, relative to the indicators in Group 2. Conclusion. The effectiveness of metabolic therapy for acute liver injury can be increased by administering succinic acid preparations together with direct-acting antioxidants, such as vitamin E and ascorbic and lipoic acids.