The effect of the extractant on the yield of biologically active substances from the leaves of Elaeagnus argentea

Abstract

The aim of the study was to study the effect of the extractant on the completeness of the yield of biologically active substances in the development of an extraction form based on the leaves of Elaeagnus argentea. Materials and methods. The objects of the study were extracts of Elaeagnus argentea leaves obtained in a ratio of 1:5 by infusing in a water bath at a temperature of 60 °C for 2 hours with periodic stirring. Purified water and ethyl alcohol in concentrations of 40, 50, 60, 80 and 96 % were used as extractants. The completeness of the BAS yield was estimated by the “dry residue” indicator and the amount of flavonoids and saponins in terms of luteolin-7-glucoside (CAS No. 5373-11-5, purity of the base substance more than 98 %) and oleanolic acid (CAS No. 508-02-1, purity of the base substance more than 99 %), respectively. Results. As a result of the study, it was found that the highest dry residue index, the amount of scrip of flavonoids and saponins were determined in the extraction, where ethyl alcohol of 60% concentration was used as an extractant. Conclusion. Thus, when extracting based on the leaves of Elaeagnus argentea, it was found that the optimal extractant is ethyl alcohol 60 %, when used, the highest value of the "dry residue" indicator and the amount of flavonoids and saponins are noted.